introductory experiments on biomolecules and their interactions

Introductory Experiments On Biomolecules And Their Interactions
Author: Robert K. Delong
Publisher: Academic Press
Release Date: 2015-03-06
Pages: 126
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Introductory Experiments on Biomolecules and their Interactions provides a novel approach to teaching biomolecules in the lab. While featuring the requisite fundamentals, it also captures the author’s experience in industry, thus providing unique, up-to-date experiments which take the learning experience one-step further. The text parallels lectures using a standard biochemistry undergraduate text. Unlike most current lab manuals available in the market which simply emphasize an introduction of techniques, this lab manual provides students with opportunities to demonstrate and prove the knowledge and theories they learn from class. Features quantitative analysis of RNA degradation by RNase Contains problem sets, calculations, and references for each lab fully immersing students in the learning process Includes instruction on how to maintain a lab notebook and write a formal lab report Provides hands-on engagement with the four major types of biomolecules and “real-life and better applied examples of molecular interactions

An Introduction To Single Molecule Biophysics
Author: Yuri L. Lyubchenko
Publisher: CRC Press
Release Date: 2017-11-22
Pages: 240
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications. Covers the most widely used single molecule biophysics techniques Provides numerous examples of current biological applications Includes practical tips on measurement optimization, resolution limits, etc. Yuri L. Lyubchenko is Professor of Pharmaceutical Sciences at the University of Nebraska Medical Center.

The Smallest Biomolecules  Diatomics And Their Interactions With Heme Proteins
Author: Abhik Ghosh
Publisher: Elsevier
Release Date: 2011-10-13
Pages: 614
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

This is not a book on NO biology, nor about hemoglobin, nor about heme-based sensors per se. Of course, it covers all these topics and more, but above all, it aims at providing a truly multidisciplinary perspective of heme-diatomic interactions. The overarching goal is to build bridges among disciplines, to bring about a meeting of minds. The contributors to this book hail from diverse university departments and disciplines – chemistry, biochemistry, molecular biology, microbiology, zoology, physics, medicine and surgery, bringing with them very different views of heme-diatomic interactions. The hope is that the juxtaposition of this diversity will lead to increased exchanges of ideas, approaches, and techniques across traditional disciplinary boundaries. The authors represent a veritable Who’s Who of heme protein research and include John Olson, Tom Spiro, Walter Zumft, F. Ann Walker, Teizo Kitagawa, W. Robert Scheidt, Pat Farmer, Marie-Alda Gilles-Gonzalez, and many other equally distinguished scientists. Extremely distinguished list of authors Multidisciplinary character – equally suitable for chemists and biochemists Covers the hottest topics in heme protein research: sensors, NO biology, new roles of hemoglobin, etc.

Metallointercalators
Author: Janice Aldrich-Wright
Publisher: Springer
Release Date: 2011-04-06
Pages: 427
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

A comprehensive treatment of the characterisation techniques used in investigating inorganic and organic molecules that interact with biomolecules is presented to the reader in a clear fashion. The work consists of two parts: (i) synthetic aspects of metallointercalators along with targeting and improving transport and (ii) the various techniques that are used for probing their interactions, such as; DNA-NMR, PGSE-NMR, DNA ESI-MS, Linear and Circular Dichroism, Fluorescence Spectroscopy, Confocal Microscopy, Viscosity, TGA and dialysis, Microarrays, biological analysis. Chapters are devoted to the synthesis and the techniques used to study the interactions of inorganic complexes with biomolecules. Considerably detailed examples are used to help illustrate the application of these techniques. This book is a useful resource for an array of inorganic and organic advanced undergraduate and graduate courses and for researchers in drug discovery.

Real Time Analysis Of Biomolecular Interactions
Author: K. Nagata
Publisher: Springer Science & Business Media
Release Date: 2013-12-11
Pages: 256
ISBN:
Available Language: English, Spanish, And French
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The metabolism of all living organisms consists of elaborate and complex interactions among numbers of biomolecules, including protein-protein interactions. BIACORE is a new device that uses surface plasmon resonance for kinetic analysis of molecular interactions and automates whole analytical processes. The number of users of BIACORE is increasing worldwide, but until now nothing had been published in English to explain the principles of this new methodology and the kinds of applications it makes available. This updated and revised version of the Japanese original explains the underlying principles of BIACORE, providing concrete examples that utilize the methodology for analysis in areas ranging from basic to applied sciences. With its generous use of illustrations, this book is a valuable source of information for all users of BIACORE.

Handbook Of Biophotonics
Author: Jürgen Popp
Publisher: John Wiley & Sons
Release Date: 2011-11-30
Pages: 1131
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

This debut edition of Visualizing Physical Geography encompasses the science of physical geography from local to global scales, using a uniquely visual approach to take students on a journey from the top layers of the Earth’s atmosphere to the rocks underlying the ocean basins to the forests of the farthest continents. As students explore the critical topics of physical geography, their study of the climate, surface features, and habitats around the world uses basic concepts of ecology, geology, chemistry, environmental science, biology, and physics and many other disciplines. Visualizing Physical Geography reinforces these interacting components and, with its premier art program, vividly illustrates the interconnectedness of physical processes that weave together to create our planet’s dynamic surface and atmosphere. Visualizing Geography relies heavily on other visuals with text to elucidate concepts for students and solidify their understanding of them. The goal is to help students understand the world around them and interpret what they see in a meaningful, accurate and exciting way. The content, design and layout of the text takes advantage of the full capacity in which students process information – visual as well as verbal. Visualizing Geography also helps students examine their own personal studying and learning styles with several new pedagogical aids –– encouraging students to apply what they are learning to their everyday lives. Visualizing Geography continues to offer ongoing study tips and psychological techniques for mastering the material. Most importantly students are provided with numerous opportunities to immediately access their understanding.

Quantifying Interactions Of Biomolecules With Inorganic Surfaces
Author: Abhijeet Patra
Publisher: Springer
Release Date: 2016-12-01
Pages: 102
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

This thesis demonstrates the adaptation of existing techniques and principles towards enabling clean and precise measurements of biomolecules interacting with inorganic surfaces. In particular, it includes real-time measurement of serum proteins interacting with engineered nanomaterial. Making meaningful and unambiguous measurements has been an evolving problem in the field of biology and its various allied domains, primarily due to the complex nature of experiments and the large number of possible interferants. The subsequent quantification of interactions between biomolecules and inorganic surfaces solves pressing problems in the rapidly developing fields of lipidomics and nanomedicine.

Biomolecules In Organic Solvents
Author: Armando Gomez-Puyou
Publisher: CRC Press
Release Date: 1992-01-27
Pages: 288
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Biomolecules and Organic Solvents discusses the behavior of enzymes and multi-enzyme complexes in organic solvents, in addition to the problem of water-protein interactions and the characteristics of water at interphases. It attempts to bridge the gap between the all water and the organic solvents systems from the point of view of enzyme structure and function. The mechanism of action of enzymes dispersed in anhydrous organic solvents and the biotechnological perspectives of the field are evaluated, and new information regarding the function and characteristics of complex multi-enzymatic systems and whole cells in organic solvents are examined. New developments regarding the nature of the water pool and interphases of reverse micelles and their enzyme kinetics are also explored. The characteristics and properties of enzymes trapped in reverse micelles are discussed throughout the book. Biomolecules in Organic Solvents is essential reading for biochemists, molecular biologists, and others working in related fields.

Photophysics Of Ionic Biochromophores
Author: Steen Brøndsted Nielsen
Publisher: Springer Science & Business Media
Release Date: 2013-10-16
Pages: 230
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

This book provides a concise overview of the photophysics and spectroscopy of bio chromophore ions. The book "Photophysics of Ionic Biochromophores" summarizes important recent advances in the spectroscopy of isolated biomolecular ions in vacuo, which has within the last decade become a highly active research field. Advanced instrumental apparatus and the steady increase in more and more powerful computers have made this development possible, both for experimentalists and theoreticians. Applied techniques described here include absorption and fluorescence spectroscopy, which are excellent indicators of environmental effects and can thus shed light on the intrinsic electronic structures of ions without perturbations from e.g. water molecules, counter ions, nearby charges, and polar amino acid residues. When compared with spectra of the chromophores in their natural environment, such spectra allow to identify possible perturbations. At the same time gas-phase spectra provide important benchmarks for quantum chemistry calculations of electronically excited states. This volume focuses on biological systems from protein biochromophores, e.g. the protonated Schiff-base retinal responsible for vision, and individual aromatic amino acids to peptides and whole proteins, studied using visible, ultraviolet and vacuum ultraviolet light. Work on DNA nucleotides and strands that are amenable to mass spectrometric studies because of the negatively charged sugarphosphate backbone are also presented. DNA strands represent an example of the interplay between multiple chromophores, which is even harder to model correctly than just single chromophores due to spatially extended excited states and weak coupling terms. The experimental techniques used to measure spectra and commonly used theoretical methods are described with a discussion on limitations and advantages. The volume includes an updated status of the field and interesting future directions such as cold ion spectroscopy.

Aspects Of Physical Biology
Author: Giancarlo Franzese
Publisher: Springer Science & Business Media
Release Date: 2008-07-10
Pages: 233
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

The application to Biology of the methodologies developed in Physics is attracting an increasing interest from the scientific community. It has led to the emergence of a new interdisciplinary field, called Physical Biology, with the aim of reaching a better understanding of the biological mechanisms at molecular and cellular levels. Statistical Mechanics in particular plays an important role in the development of this new field. For this reason, the XXth session of the famous Sitges Conference on Statistical Physics was dedicated to "Physical Biology: from Molecular Interactions to Cellular Behavior". As is by now tradition, a number of lectures were subsequently selected, expanded and updated for publication as lecture notes, so as to provide both a state-of-the-art introduction and overview to a number of subjects of broader interest and to favor the interchange and cross-fertilization of ideas between biologists and physicists. The present volume focuses on three main subtopics (biological water, protein solutions as well as transport and replication), presenting for each of them the on-going debates on recent results. The role of water in biological processes, the mechanisms of protein folding, the phases and cooperative effects in biological solutions, the thermodynamic description of replication, transport and neural activity, all are subjects that are revised in this volume, based on new experiments and new theoretical interpretations.

NMR For Chemists And Biologists
Author: Rodrigo J Carbajo
Publisher: Springer Science & Business Media
Release Date: 2013-06-24
Pages: 115
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

This book intends to be an easy and concise introduction to the field of nuclear magnetic resonance or NMR, which has revolutionized life sciences in the last twenty years. A significant part of the progress observed in scientific areas like Chemistry, Biology or Medicine can be ascribed to the development experienced by NMR in recent times. Many of the books currently available on NMR deal with the theoretical basis and some of its main applications, but they generally demand a strong background in Physics and Mathematics for a full understanding. This book is aimed to a wide scientific audience, trying to introduce NMR by making all possible effort to remove, without losing any formality and rigor, most of the theoretical jargon that is present in other NMR books. Furthermore, illustrations are provided that show all the basic concepts using a naive vector formalism, or using a simplified approach to the particular NMR-technique described. The intention has been to show simply the foundations and main concepts of NMR, rather than seeking thorough mathematical expressions.

Charge Transfer Interactions Of Biomolecules
Author: Michael A. Slifkin
Publisher:
Release Date: 1971
Pages: 271
ISBN:
Available Language: English, Spanish, And French
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Computational Methods To Study The Structure And Dynamics Of Biomolecules And Biomolecular Processes
Author: Adam Liwo
Publisher: Springer Science & Business Media
Release Date: 2013-07-17
Pages: 810
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Since the second half of the 20th century machine computations have played a critical role in science and engineering. Computer-based techniques have become especially important in molecular biology, since they often represent the only viable way to gain insights into the behavior of a biological system as a whole. The complexity of biological systems, which usually needs to be analyzed on different time- and size-scales and with different levels of accuracy, requires the application of different approaches, ranging from comparative analysis of sequences and structural databases, to the analysis of networks of interdependence between cell components and processes, through coarse-grained modeling to atomically detailed simulations, and finally to molecular quantum mechanics. This book provides a comprehensive overview of modern computer-based techniques for computing the structure, properties and dynamics of biomolecules and biomolecular processes. The twenty-two chapters, written by scientists from all over the world, address the theory and practice of computer simulation techniques in the study of biological phenomena. The chapters are grouped into four thematic sections dealing with the following topics: the methodology of molecular simulations; applications of molecular simulations; bioinformatics methods and use of experimental information in molecular simulations; and selected applications of molecular quantum mechanics. The book includes an introductory chapter written by Harold A. Scheraga, one of the true pioneers in simulation studies of biomacromolecules.

Electrical Interactions In Molecular Biophysics
Author: Raymond Gabler
Publisher: Elsevier
Release Date: 2012-12-02
Pages: 364
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Electrical Interactions in Molecular Biophysics: An Introduction deals with electrical interactions between biomolecules and therefore encompasses two disciplines, molecular biology and physics. The emphasis is on the electrical nature of biochemical or molecular biological reactions. The principles of electrostatics are used to explain some of the basic units of structure on a molecular level. Comprised of nine chapters, this book opens with an overview of the concepts and structures of biochemistry, with particular reference to different structural biochemical groups and how they are used as building blocks in forming molecules. The following chapters discuss the basics of elementary electrostatics; dielectric constants and dipoles; the dipole moments of biomolecules; van der Waals forces; and Debye-Huckel theory. Water and water structure are also considered from a physical standpoint. The final chapter is devoted to experimental techniques that rely upon the electrical properties of biomolecules and explains what types of information can be obtained from each experimental form. This monograph will be of interest to students and practitioners in biochemistry, molecular biology, biophysics, or microbiology.

Non Covalent Interactions
Author: Pavel Hobza
Publisher: Royal Society of Chemistry
Release Date: 2010
Pages: 225
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Co-authored by an experimentalist (Klaus M ller-Dethlefs ) and theoretician (Pavel Hobza), the aim of this book is to provide a general introduction into the science behind non-covalent interactions and molecular complexes using some important experimental and theoretical methods and approaches."

Dynamic Force Spectroscopy And Biomolecular Recognition
Author: Anna Rita Bizzarri
Publisher: CRC Press
Release Date: 2012-01-25
Pages: 270
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Molecular recognition, also known as biorecognition, is the heart of all biological interactions. Originating from protein stretching experiments, dynamic force spectroscopy (DFS) allows for the extraction of detailed information on the unbinding process of biomolecular complexes. It is becoming progressively more important in biochemical studies and is finding wider applications in areas such as biophysics and polymer science. In six chapters, Dynamic Force Spectroscopy and Biomolecular Recognition covers the most recent ideas and advances in the field of DFS applied to biorecognition: Chapter 1: Reviews the basic and novel aspects of biorecognition and discusses the emerging capabilities of single-molecule techniques to disclose kinetic properties and molecular mechanisms usually hidden in bulk measurements Chapter 2: Describes the basic principle of atomic force microsocopy (AFM) and DFS, with particular attention to instrumental and theoretical aspects more strictly related to the study of biomolecules Chapter 3: Overviews the theoretical background in which experimental data taken in nonequilibrum measurements of biomolecular unbinding forces are extrapolated to equilibrium conditions Chapter 4: Reviews the most common and efficient strategies adopted in DFS experiments to immobilize the interacting biomolecules to the AFM tip and to the substrate Chapter 5: Presents and discusses the most representative aspects related to the analysis of DFS data and the challenges of integrating well-defined criteria to calibrate data in automatic routinary procedures Chapter 6: Overviews the most relevant DFS applications to study biorecognition processes, including the biotin/avidin pair, and selected results on various biological complexes, including antigen/antibody, proteins/DNA, and complexes involved in adhesion processes Chapter 7: Summarizes the main results obtained by DFS applied to study biorecognition processes with forthcoming theoretical and experimental advances Although DFS is a widespread, worldwide technique, no books focused on this subject have been available until now. Dynamic Force Spectroscopy and Biomolecular Recognition provides the state of the art of experimental data analysis and theoretical procedures, making it a useful tool for researchers applying DFS to study biorecognition processes.

Physical Properties And Thermodynamic Behaviour Of Minerals
Author: Ekhard K.H. Salje
Publisher: Springer Science & Business Media
Release Date: 1988-01-31
Pages: 707
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

The role played by earth sciences in the scientific community has changed considerably during this century. Since the revolutionary discoveries of global processes such as plate tectonics, there has been an increasing awareness of just how fundamental many of the mechanisms which dominate in these processes depend on the physical properties of the materials of which the earth is made. One of the prime objectives of mineral sciences is now to understand and predict these properties in a truly quantitative manner. The macroscopic properties which are of most immediate interest in this context fall within the conventional definitions of thermodynamics, magnetism, elasticity, dielectric susceptibilities, conductivity etc. These properties reflect the microscopic contributions, at an atomistic level, of harmonic and anharmonic lattice vibrations, ionic and electronic transport as well as a great variety of ordering and clustering phenomena. The advances made by solid state physicists and chemists in defining the underlying phenomena lnvolved in the thermal evolution of materials have stimulated major new research initiatives within the Earth Sciences. Earth Scientists have combined to form active groups within the wider community of solid state and materials scientists working towards a better understanding of those physical processes which govern not only the behaviour of simple model compounds but also that of complex materials like minerals. Concomitant with this change in direction has come an increasing awareness of the need to use the typical working tools of other disciplines.

General Catalog
Author: Orta Doğu Teknik Üniversitesi (Ankara, Turkey)
Publisher:
Release Date: 2002
Pages:
ISBN:
Available Language: English, Spanish, And French
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Statistical Physics Of Biomolecules
Author: Daniel M. Zuckerman
Publisher: CRC Press
Release Date: 2010
Pages: 334
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

"It is essential for modern students of molecular behavior to understand the statistical/chemical physics at the heart of modern molecular science. But traditional presentations of this material are often difficult to penetrate. This volume brings down to earth some of the most intimidating but important theories of molecular biophysics. Students build understanding by focusing on topics such as probability theory, low-dimensional models, and the simplest molecular systems. The book's accessible development of equilibrium and dynamical statistical physics makes this a valuable text for students with limited physics and chemistry backgrounds."--

Trends In Drug Research III
Author: H. van der Goot
Publisher: Elsevier
Release Date: 2002-01-25
Pages: 272
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

The tradition of setting new trends in medicinal chemistry continued at the 13th Symposium where topics included chemical and biological diversity, new paradigms in drug action, and new insights in receptor mechanisms. Other topics of great interest discussed, and included in these proceedings, are the discoveries in green chemistry, the interface between organic synthesis and biosynthesis, the growing problem of resistant micro-organisms and the possibilities to identify new, and better, antibiotics. And finally, in recent developments, the discovery of small molecules with insulin sensitizing properties.