introduction to protein mass spectrometry

Introduction To Protein Mass Spectrometry
Author: Pradip Kumar Ghosh
Publisher: Academic Press
Release Date: 2015-12-10
Pages: 312
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Introduction to Protein Mass Spectrometry provides a comprehensive overview of this increasingly important, yet complex, analytical technique. Unlike many other methods which automatically yield an absolutely unique protein name as output, protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products. This book enables readers to both understand, and appreciate, how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources. Citing the most recent and relevant work in the field of biological mass spectrometry, the book is written for researchers and scientists new to the field, but is also an ideal resource for those hoping to hone their analytical abilities. Offers introductory information for scientists and researchers new to the field, as well as advanced insight into the critical assessment of computer-analyzed mass spectrometric results and their current limitations Provides examples of commonly-used MS instruments from Bruker, Applied Biosystems, JEOL, Thermo Scientific/Thermo Fisher Scientific, IU, and Waters Includes biological applications and exploration of analytical tools and databases for bioinformatics

Protein Mass Spectrometry
Author: Julian Whitelegge
Publisher: Elsevier Science
Release Date: 2009
Pages: 552
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

This book is designed to be a central text for young graduate students interested in mass spectrometry as it relates to the study of protein structure and function as well as proteomics. It is a definite must-have work for: - libraries at academic institutions with Master and Graduate programs in biochemistry, molecular biology, structural biology and proteomics - individual laboratories with interests covering these areas - libraries and individual laboratories in the pharmaceutical and biotechnology industries. *Serves as an essential reference to those working in the field *Incorporates the contributions of prominent experts *Features comprehensive coverage and a logical structure

Characterization Of Protein Therapeutics Using Mass Spectrometry
Author: Guodong Chen
Publisher: Springer Science & Business Media
Release Date: 2014-07-08
Pages: 404
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

This book highlights current approaches and future trends in the use of mass spectrometry to characterize protein therapies. As one of the most frequently utilized analytical techniques in pharmaceutical research and development, mass spectrometry has been widely used in the characterization of protein therapeutics due to its analytical sensitivity, selectivity, and specificity. This book begins with an overview of mass spectrometry techniques as related to the analysis of protein therapeutics, structural identification strategies, quantitative approaches, followed by studies involving characterization of process related protein drug impurities/degradants, metabolites, higher order structures of protein therapeutics. Both general practitioners in pharmaceutical research and specialists in analytical sciences will benefit from this book that details step-by-step approaches and new strategies to solve challenging problems related to protein therapeutics research and development.

Protein And Peptide Analysis By Mass Spectrometry
Author: John R. Chapman
Publisher: Springer
Release Date: 1996-01-01
Pages: 350
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Leading practitioners authoritatively describe the newest and most effective spectrometric techniques for the analysis of proteins and peptides. The areas covered range from the elucidation of primary and secondary protein structure and the rapid identification of proteins using database techniques to methods for sequencing, as well as methods for the quantitative determination of peptides. Other chapters provide detailed information on the analysis of glycoproteins and glycopeptides and on the use of mass spectrometry to probe the interactions of proteins, both covalent and noncovalent.

Protein Sequencing And Identification Using Tandem Mass Spectrometry
Author: Michael Kinter
Publisher: John Wiley & Sons
Release Date: 2005-04-12
Pages: 320
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

How to design, execute, and interpret experiments for proteinsequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases inrecent years has triggered an explosive growth in the applicationof mass spectrometry to protein sequencing. This timely andauthoritative book provides professionals and scientists inbiotechnology research with complete coverage of procedures foranalyzing protein sequences by mass spectrometry, includingstep-by-step guidelines for sample preparation, analysis, and datainterpretation. Michael Kinter and Nicholas Sherman present their ownhigh-quality, laboratory-tested protocols for the analysis of awide variety of samples, demonstrating how to carry out specificexperiments and obtain fast, reliable results with a 99% successrate. Readers will get sufficient experimental detail to apply intheir own laboratories, learn about the proper selection andoperation of instruments, and gain essential insight into thefundamental principles of mass spectrometry and protein sequencing.Coverage includes: Peptide fragmentation and interpretation of product ionspectra Basic polyacrylamide gel electrophoresis Preparation of protein digests for sequencing experiments Mass spectrometric analysis using capillary liquidchromatography Techniques for protein identification by database searches Characterization of modified peptides using tandem massspectrometry And much more

Wnt Signaling In Development And Disease
Author: Stefan P. Hoppler
Publisher: John Wiley & Sons
Release Date: 2014-02-28
Pages: 472
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Wnt signaling in Development and Disease: Molecular Mechanisms and Biological Functions reviews the core topics in Wnt signaling, from molecular pathway mechanisms to its role in embryogenesis, adult tissue homeostasis, and chronic disease. Written by a team of expert reviewers, the book provides clear and concise coverage of the core foundations of Wnt signaling before advancing to discussion of cutting-edge scientific research. Focused on the biological insights and current scientific questions of Wnt signaling, this book will be a comprehensive and definitive resource for a wide range of researchers and students in cell signaling, cell physiology, developmental biology, and biomedical engineering, as well as anyone interested in learning more about this important and complex protein network. • A definitive source of information on Wnt signaling and its role in development and disease, written by leaders in the field. • Explores the role of Wnt signaling in chronic disease such as melanoma, colorectal cancer, dementia, and psychiatric diseases • Reviews the complex processes of signal integration and regulation • Features broad discussion of Wnt signaling biology as well as detailed discussion of the pathway’s role in diseases and potential clinical applications.

Introduction To Protein Science
Author: Arthur Lesk
Publisher: Oxford University Press
Release Date: 2010-03-25
Pages: 455
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Introduction to Protein Science provides a broad introduction to the contemporary study of proteins in health and disease, suitable for students on biological, biochemical, and biomedical degrees internationally. The book relates the study of proteins to the context of modern high-throughput data streams of genomics and proteomics.

Introduction To Mass Spectrometry
Author: J. Throck Watson
Publisher: John Wiley & Sons
Release Date: 2013-07-09
Pages: 864
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Completely revised and updated, this text provides an easy-to-read guide to the concept of mass spectrometry and demonstrates its potential and limitations. Written by internationally recognised experts and utilising "real life" examples of analyses and applications, the book presents real cases of qualitative and quantitative applications of mass spectrometry. Unlike other mass spectrometry texts, this comprehensive reference provides systematic descriptions of the various types of mass analysers and ionisation, along with corresponding strategies for interpretation of data. The book concludes with a comprehensive 3000 references. This multi-disciplined text covers the fundamentals as well as recent advance in this topic, providing need-to-know information for researchers in many disciplines including pharmaceutical, environmental and biomedical analysis who are utilizing mass spectrometry

Liquid Chromatography Mass Spectrometry
Author: Wilfried M.A. Niessen
Publisher: CRC Press
Release Date: 2006-08-09
Pages: 632
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

A constructive evaluation of the most significant developments in liquid chromatography-mass spectrometry (LC-MS) and its uses for quantitative bioanalysis and characterization for a diverse range of disciplines, Liquid Chromatography-Mass Spectrometry, Third Edition offers a well-rounded coverage of the latest technological developments and

Protein And Peptide Mass Spectrometry In Drug Discovery
Author: Michael L. Gross
Publisher: John Wiley & Sons
Release Date: 2011-09-26
Pages: 484
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

The book that highlights mass spectrometry and its application in characterizing proteins and peptides in drug discovery An instrumental analytical method for quantifying the mass and characterization of various samples from small molecules to large proteins, mass spectrometry (MS) has become one of the most widely used techniques for studying proteins and peptides over the last decade. Bringing together the work of experts in academia and industry, Protein and Peptide Mass Spectrometry in Drug Discovery highlights current analytical approaches, industry practices, and modern strategies for the characterization of both peptides and proteins in drug discovery. Illustrating the critical role MS technology plays in characterizing target proteins and protein products, the methods used, ion mobility, and the use of microwave radiation to speed proteolysis, the book also covers important emerging applications for neuroproteomics and antigenic peptides. Placing an emphasis on the pharmaceutical industry, the book stresses practice and applications, presenting real-world examples covering the most recent advances in mass spectrometry, and providing an invaluable resource for pharmaceutical scientists in industry and academia, analytical and bioanalytical chemists, and researchers in protein science and proteomics.

Introduction To Peptides And Proteins
Author: Ulo Langel
Publisher: CRC Press
Release Date: 2009-11-18
Pages: 420
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Human cells produce at least 30,000 different proteins. Each has a specific function characterized by a unique sequence and native conformation that allows it to perform that function. While research in this post-genomic era has created a deluge of invaluable information, the field has lacked for an authoritative introductory text needed to inform researchers and students in all of those fields now concerned with protein research. Introduction to Peptides and Proteins brings together some of the most respected researchers in protein science to present a remarkably coherent introduction to modern peptide and protein chemistry. The first sections of the book delve into – Basic peptide and protein science from assembly through degradation Traditional and emerging research methods including those used in bioinformatics and proteomics New computational approaches and algorithms used to find patterns in the vast data collected by sequencing projects After providing a foundation in tools and methods, the authors closely examine six protein families, including representative classes such as enzymes, cell-surface receptors, antibodies, fibrous proteins, and bioactive peptide classes. They concentrate on biochemical mechanisms and where possible indicate therapeutic or biotechnical possibilities. Then focusing on clinical aspects, the authors investigate misfolding as found in prion diseases, miscleavage as found in Alzheimer’s, and mis-sequencing as found with some cancers. Drawing from some of their own research, the authors summarize recent achievements and emerging applications. They discuss the use of proteins and peptides as drugs and the solid-phase synthesis required for drug production. They also look at the use of peptides as functional biomolecules and research tools. No longer just the domain of biologists, many key advances in protein research started in physics labs and have involved contributions from a host of fields including statistics, drug development, genetics, and chemical spectroscopy. Introduction to Peptides and Proteins provides researchers across these fields with the thorough foundation needed to explore all the potential that protein research offers.

Acceleration And Improvement Of Protein Identification By Mass Spectrometry
Author: Willy Vincent Bienvenut
Publisher: Springer Science & Business Media
Release Date: 2005-04-19
Pages: 298
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

At present where protein identification and characterisation using mass spectrometry is a method of choice, this book is presenting a review of basic proteomic techniques. The second part of the book is related to the novel high throughput protein identification technique called the 'molecular scanner'. Several protein identification techniques are described, especially the peptide mass fingerprint with MALDI-MS based method. E.g. ionisation process, matrix available, signal reproducibility and suppression effect, as well as date treatment for protein identification using bioinformatics tools.

Mass Spectrometry In Drug Metabolism And Disposition
Author: Mike S. Lee
Publisher: John Wiley & Sons
Release Date: 2011-03-16
Pages: 800
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

This book examines the background, industrial context, process, analytical methodology, and technology of metabolite identification. It emphasizes the applications of metabolite identification in drug research. While primarily a textbook, the book also functions as a comprehensive reference to those in the industry. The authors have worked closely together and combine complementary backgrounds to bring technical and cultural awareness to this very important endeavor while serving to address needs within academia and industry It also contains a variety of problem sets following specific sections in the text.

Mass Spectrometry And Hyphenated Techniques In Neuropeptide Research
Author: Jerzy Silberring
Publisher: John Wiley & Sons
Release Date: 2002-05-02
Pages: 558
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

The first authoritative guide to the application of this vital analytical technique Mass spectrometry is a powerful analytical technique that is used to identify unknown compounds, to quantify known materials, and to elucidate the structural and chemical properties of molecules. In analyzing the effects of experimental drugs on the brain, it is the sole technique for identifying the presence and structure of neuropeptides-substances that indicate the effect of the drug. Mass Spectrometry and Hyphenated Techniques in Neuropeptide Research specifically explains how to apply the technology to this process. Because the book is written by mass spectrometry users, as opposed to mass spectrometrists, the focus remains on practical applications of the technique. The authors demonstrate how mass spectrometry works, how to apply the technique to research, which types of instrumentation should be used for particular requirements, and how to plan experiments. Readers will learn why mass spectrometry provides more outcome features than other techniques in neuropeptide analysis, including simultaneous detection, identification of substances present in mixtures, and sequence information even when the residues are modified, blocked, or unusual. Among the chapters in this comprehensive text are: * Sequencing of Peptides by Nanospray Mass Spectrometry * Laser-Machined Microdevices for Mass Spectrometry * Electron Capture Dissociation of Peptides * Synthesis of Combinatorial Peptide Libraries * Analysis of Tissues That Reflect Nervous System Disease Doctoral students, researchers, and industry professionals in pharmacology, chemistry, biochemistry/biotechnology, and medicine will find Mass Spectrometry and Hyphenated Techniques in Neuropeptide Research to be an indispensable starting point for understanding peptides, their function, and identification.

Mass Spectrometry For Microbial Proteomics
Author: Haroun N. Shah
Publisher: John Wiley & Sons
Release Date: 2010-10-28
Pages: 534
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

New advances in proteomics, driven largely by developments in mass spectrometry, continue to reveal the complexity and diversity of pathogenic mechanisms among microbes that underpin infectious diseases. Therefore a new era in medical microbiology is demanding a rapid transition from current procedures to high throughput analytical systems for the diagnosis of microbial pathogens. This book covers the broad microbiological applications of proteomics and mass spectrometry. It is divided into six sections that follow the general progression in which most microbiology laboratories are approaching the subject –Transition, Tools, Preparation, Profiling by Patterns, Target Proteins, and Data Analysis.

Mass Spectrometry And Nutrition Research
Author: Laurent Bernard Fay
Publisher: Royal Society of Chemistry
Release Date: 2010
Pages: 339
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Mass spectrometry has developed into a platform for the assessment of health, sensory, quality and safety aspects of food. Current nutrition research focuses on unravelling the link between acute or chronic dietary and nutrient intake and the physiological effects at cellular, tissue and whole body level. The bioavailability and bioefficacy of food constituents and dose-effect correlations are key to understanding the impact of food on defined health outcomes. To generate this information, appropriate analytical tools are required to identify and quantify minute amounts of individual compounds in highly complex matrices (such as food or biological fluids) and to monitor molecular changes in the body in a highly specific and sensitive manner. Mass spectrometry has become the method of choice for such work and now has broad applications throughout all areas of nutrition research. This book focuses the contribution of mass spectrometry to the advancement of nutrition research. Aimed and students, teachers and researchers, it provides a link between nutrition and analytical biochemistry. It guides nutritionists to the appropriate techniques for their work and introduces analytical biochemists to new fields of application in nutrition and health. The first part of the book is dedicated to the assessment of macro- and micro-nutrient status with a view to making dietary recommendations for the treatment of diet-related diseases. The second part shows how mass spectrometry has changed nutrition research in fields like energy metabolism, body composition, protein turnover, immune modulation and cardiovascular health.

Proteome Research  Mass Spectrometry
Author: Peter James
Publisher: Springer Science & Business Media
Release Date: 2000-10-26
Pages: 274
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Recent advances in large scale DNA sequencing technology have made it possible to sequence the entire genome of an organism. Attention is now turning to the analysis of the product of the genome, the proteome, which is the set of proteins being expressed by a cell. Mass spectrometry is the method of choice for the rapid large-scale identification of these proteomes and their modifications. This is the first book to extensively cover the applications of mass spectrometry to proteome research.

Mass Spectrometry Data Analysis In Proteomics
Author: Rune Matthiesen
Publisher: Springer Science & Business Media
Release Date: 2007
Pages: 320
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

The intention of Mass Spectrometry Data Analysis in Proteomics is to support researchers in deciding which programs to use in various tasks related to analysis of mass spectrometry data in proteomics. The chapters give a precise description of the theoretical background of each topic followed accurate descriptions of programs and the parameters best suited for different cases.

Mass Spectrometry  Modified Proteins And Glycoconjugates
Author: A.L. Burlingame
Publisher: Gulf Professional Publishing
Release Date: 2005-12-27
Pages: 432
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

This volume provides comprehensive treatment of tools and proper usage for the identification of proteins, affinity chromatography and studies the complexity of protein machines and assemblages, assignment of the most common protein posttranslational modifications (phosphorylation and glycosylation) and glycolipidomics. *Part 2 of 2 volumes about Mass Spectrometry *Discusses peptide and protein cleanup and preparation requirements for mass spectrometry *Explains protein enzymic and chemical digestion strategies *Includes case studies of protein assemblages and machines

Introduction To Proteomics
Author: Daniel Liebler
Publisher: Springer Science & Business Media
Release Date: 2001-12-04
Pages: 198
ISBN:
Available Language: English, Spanish, And French
EBOOK SYNOPSIS:

Daniel C. Liebler masterfully introduces the science of proteomics by spelling out the basics of how one analyzes proteins and proteomes, and just how these approaches are then employed to investigate their roles in living systems. He explains the key concepts of proteomics, how the analytical instrumentation works, what data mining and other software tools do, and how these tools can be integrated to study proteomes. Also discussed are how protein and peptide separation techniques are applied in proteomics, how mass spectrometry is used to identify proteins, and how data analysis software enables protein identification and the mapping of modifications. In addition, there are proteomic approaches for analyzing differential protein expression, characterizing proteomic diversity, and dissecting protein-protein interactions and networks.